Regulatory

Part:BBa_M36177:Design

Designed by: Majed Magzoub, Guhan Venkataraman, Joe Carpenter   Group: Stanford BIOE44 - S11   (2015-10-23)


pH sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 386
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We were limited by the following restrictions (1) 200-1500 bp size limit, (2) no Bbsl, Bsal, or BsmBI sites, (3) no repeats of length greater than 5 base pairs.


Source

rcFB transcriptional regulator in Lactococcus lactis genome.

References